Intro to Molecular, Cellular and Developmental Biology: Spring 2010

A microbiologist discovers a new type II restriction endonuclease. When DNA is digested by this enzyme, fragments that average 1,048,500 bp in length are produced. What is the most likely number of base pairs in the recognition sequence of this enzyme?

Two restriction endonucleases (EcoRI and BamHI) are used to cut a piece of linear DNA, singly and in pairwise combination into the following fragments (sizes are in kb). Determine the correct order of restriction sites, and draw the map, with the intervals between sites labeled.

Two restriction endonucleases (HaeIII and PstI) are used to cut a piece of circular DNA, singly and in pairwise combination into the following fragments (sizes are in kb). Determine the correct order of restriction sites, and draw the map, with the intervals between sites labeled.