Biol Concepts Spring 04 sec 001,,,,,,,,,,,,,,,, Absorbance values for spinach extract pigments,,,,,,,,,,,,,,,, chl a,,,,,,,,,,,,,,,, ,400,420,440,460,480,500,520,540,560,580,600,620,640,660,680,700 I,0.216,0.283,0.212,0.04,0.038,0.031,0.033,0.032,0.047,0.04,0.051,0.058,0.071,0.163,0.083,0.021 II,0.333,0.42,0.242,0.057,0.07,0.054,0.043,0.043,0.054,0.059,0.054,0.053,0.076,0.171,0.059,0.016 III,0.292,0.38,0.274,0.111,0.078,0.044,0.066,0.06,0.067,0.102,0.098,0.1,0.122,0.214,0.093,0.057 IV,0.63,0.89,0.488,0.108,0.066,0.033,0.035,0.041,0.048,0.064,0.083,0.106,0.134,0.396,0.139,0.017 Average,,,,,,,,,,,,,,,, chl b,,,,,,,,,,,,,,,, ,400,420,440,460,480,500,520,540,560,580,600,620,640,660,680,700 I,0.061,0.111,0.207,0.229,0.052,0,0,0,0.006,0,0.005,0.007,0.042,0.019,0.008,0 II,0.255,0.355,0.508,0.554,0.185,0.065,0.067,0.072,0.072,0.072,0.064,0.058,0.059,0.099,0.072,0.034 III,0.197,0.257,0.357,0.452,0.169,0.068,0.076,0.089,0.079,0.095,0.102,0.095,0.139,0.11,0.018,0.033 IV,0.432,0.722,1.345,1.999,0.328,0.1057,0.051,0.05,0.052,0.071,0.076,0.087,0.206,0.158,0.035,0.005 Average,,,,,,,,,,,,,,,, carotenols,,,,,,,,,,,,,,,, ,400,420,440,460,480,500,520,540,560,580,600,620,640,660,680,700 I,0.228,0.3,0.386,0.319,0.219,0.073,0.028,0.008,0.044,0.037,0.039,0.035,0.027,0.013,0.037,0.02 II,0.398,0.472,0.542,0.456,0.294,0.106,0.069,0.055,0.065,0.054,0.027,0.037,0.022,0.0247,0.023,0.024 III,0.276,0.348,0.386,0.3,0.222,0.044,0.022,0.014,0,0.018,0.03,0.02,0.018,0.02,0,0.014 IV,0.945,1.999,1.999,1.999,0.96,0.171,0.054,0.031,0.022,0.022,0.025,0.022,0.016,0.017,0.012,0.011 Average,,,,,,,,,,,,,,,, beta-carotene,,,,,,,,,,,,,,,, ,400,420,440,460,480,500,520,540,560,580,600,620,640,660,680,700 I,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0 II,0.018,0.018,0.018,0.027,0.0147,0.01,0.01,0.012,0.011,0.008,0.002,0.002,0.004,0.025,0.009,0.009 III,0,0,0.004,0.006,0.01,0.009,0,0,0,0,0.001,0,0,0,0,0 IV,0.033,0.03,0.035,0.023,0.02,0.017,0.001,0.006,0.006,0.006,0.009,0.008,0.006,0.003,0.003,0.004 Average,,,,,,,,,,,,,,,, extract,,,,,,,,,,,,,,,, ,400,420,440,460,480,500,520,540,560,580,600,620,640,660,680,700 I,1.999,1.999,1.999,1.999,1.999,1.999,1.23,1.025,0.915,0.835,0.756,0.672,0.668,0.752,0.51,0.392 II,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.74,1.5 III,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999,1.999 IV,1.999,1.999,1.999,1.999,1.999,1.999,1.66,1.21,0.985,0.915,0.84,0.772,0.82,0.995,0.592,0.442 Average,,,,,,,,,,,,,,,, Concentration of chl a and chl b,, ,chl a,chl b ,663,645 I,n/a,n/a II,0.098,0.065 III,0.223,0.109 IV,0.365,0.265 Average,,