Biol Concepts Spring 04 sec 002,,,,,,,,,,,,,,,, Absorbance values for spinach extract pigments,,,,,,,,,,,,,,,, chl a,,,,,,,,,,,,,,,, ,400,420,440,460,480,500,520,540,560,580,600,620,640,660,680,700 I,1.055,1.18,0.49,0.094,0.059,0.044,0.056,0.062,0.092,0.118,0.154,0.109,0.247,0.616,0.177,0.024 II,1.99,1.99,1.99,0.656,0.39,0.175,0.165,0.2,0.256,0.331,0.414,0.504,0.616,1.99,0.434,0.074 III,0.145,0.316,0.644,1.04,0.215,0.039,-0.063,0.034,0.041,0.061,0.069,0.088,0.23,0.13,0.018,0.013 IV,1.999,1.99,0.678,0.148,0.079,0.019,0.06,0.057,0.094,0.15,0.198,0.184,0.316,0.875,0.198,-0.002 Average,,,,,,,,,,,,,,,, chl b,,,,,,,,,,,,,,,, ,400,420,440,460,480,500,520,540,560,580,600,620,640,660,680,700 I,0.257,0.392,0.572,0.668,0.21,0.077,0.063,0.068,0.066,0.09,0.054,0.049,0.207,0.143,0.053,0.024 II,0.815,1.99,1.99,1.99,0.572,0.133,0.108,0.127,0.175,0.233,0.252,0.297,0.602,0.504,0.123,0.046 III,1.999,1.999,0.855,0.074,0.034,-0.005,-0.89,0.018,0.047,0.077,0.123,0.168,0.225,1.06,0.2,-0.011 IV,0.21,0.382,0.678,0.835,0.169,0.003,0.021,0.024,0.026,0.062,0.055,0.037,0.22,0.066,-0.033,-0.027 Average,,,,,,,,,,,,,,,, carotenols,,,,,,,,,,,,,,,, ,400,420,440,460,480,500,520,540,560,580,600,620,640,660,680,700 I,0.266,0.345,0.412,..374,0.273,0.055,0.015,0.008,0.011,0.007,0.003,-0.07,0.008,0.008,0.006,0.005 II,0.488,0.72,0.975,0.885,0.608,0.128,0.022,0.019,0.012,0.009,0.014,0.008,0.012,0.029,0.014,0.009 III,0.286,0.428,0.528,0.464,0.341,0.039,-0.092,-0.026,-0.016,-0.019,-0.012,-0.022,-0.021,-0.022,-0.022,0.004 IV,0.19,0.22,0.233,0.231,0.155,0.008,0.019,0,-0.013,-0.066,-0.009,-0.098,0,-0.017,-0.016,-0.02 Average,,,,,,,,,,,,,,,, beta-carotene,,,,,,,,,,,,,,,, ,400,420,440,460,480,500,520,540,560,580,600,620,640,660,680,700 I,0.014,0.03,0.031,0.035,0.03,0.011,0.004,0,0.001,-0.001,-0.003,-0.075,0.001,0.001,0.001,0.002 II,0.073,0.102,0.128,0.147,0.128,0.062,0.012,0.011,0.008,0.004,0.007,0.002,0.007,0.008,0.003,0.009 III,0.033,0.037,0.068,0.056,0.067,0.028,-0.086,0.007,0.005,-0.003,-0.004,-0.003,-0.005,-0.009,-0.014,-0.009 IV,0,0.016,0.026,0.042,0.028,-0.027,-0.02,-0.035,-0.043,-0.023,-0.032,-0.073,-0.039,-0.06,-0.052,-0.026 Average,,,,,,,,,,,,,,,, Concentration of chl a and chl b,,,,,,,,,,,,,,,, ,chl a,chl b,,,,,,,,,,,,,, ,663,645,,,,,,,,,,,,,, I,0.592,0.225,,,,,,,,,,,,,, II,0.16,0.43,,,,,,,,,,,,,, III,,,,,,,,,,,,,,,, IV,0.688,0.168,,,,,,,,,,,,,, Average,,,,,,,,,,,,,,,,