Key

Operating Instructions

1. Turn the instrument on by rotating the amplifier control (c) in a clock-wise direction. Allow 5 minutes for the instrument to warm up.
2. Rotate the wavelength control (a) until the desired wavelength is shown on the wavelength scale (b).
3. Adjust the amplifier control (c) with the sample compartment lid (e) closed until the meter needle reads 0 on the % Transmittance scale (d). This is zeroing the meter.
4. Fill a clean cuvette with an appropriate blank solution and wipe it with lens paper to remove liquid droplets, dust and finger prints.
5. Place the blank cuvette in the sample compartment and align the guide mark on the cuvette with the guide mark at the front of the sample compartment. Close the lid.
6. Rotate the light control (f) so that the meter reads 100 on the % Transmittance scale or 0 on the Absorbance scale.
7. Remove the blank cuvette.
8. Load the test samples in to cuvettes and wipe them.
9. Load the test cuvettes in the sample compartment and read the Absorbance or % Transmittance on the dial.

Note:

1. Steps 2-9 must be repeated whenever the wavelength is changed.
2. Change to the red phototube and red filter when the wavelength is greater than 600nm.
3. Check often to be certain that the meter needle reads 0 on the % Transmittance scale with the sample compartment empty and the lid closed.
4. Check often to be certain that the meter needle reads 100% Transmittance when the blank cuvette is in the sample compartment with the lid closed.
5. Use a matched set of cuvettes. Do not mix cuvettes of different sizes or designs.